5ZC3

The Crystal Structure of PcRxLR12

5ZC3 の概要

• エントリーDOI: 10.2210/pdb5zc3/pdb
• 分子名称: RxLR effector (1 entity in total)
• 機能のキーワード: rxlr effectors, immunosuppressant
• 由来する生物種: Phytophthora capsici
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 107110.16

構造登録者

Zhao, L.,Zhang, X.,Zhu, C. (登録日: 2018-02-14, 公開日: 2018-08-15, 最終更新日: 2024-10-30)

主引用文献

Zhao, L.,Zhang, X.,Zhang, X.,Song, W.,Li, X.,Feng, R.,Yang, C.,Huang, Z.,Zhu, C.
Crystal structure of the RxLR effector PcRxLR12 from Phytophthora capsici
Biochem. Biophys. Res. Commun., 503:1830-1835, 2018

PubMed Abstract: RxLR genes are a prominent class of effectors in oomycetes, and almost half of these proteins contain a conserved sequence motif termed the WY domain, that may exist singly, or as divergent tandem repeats in different effectors. Here we describe the crystal structure of PcRxLR12 (63-488) from Phytophthora capsici at 3.0 Å resolution. The structure consists of five tandemly arrayed WY-domains linked to each other by short connecting helices. Superposition of the WY-2 domain on the other four domains of PcRxLR12, show that the first α-helix termed the K motif, and Loop 3 which connects α and α are the key regions of structural divergence between the WY domains. A similar pattern was observed when WY-2 was superposed on the 11 WY domains from other oomycete effectors. We also note that an added connecting helix between WY domains in some RXLR effectors, ensures that the WY domains are oriented in the same direction.

PubMed: 30077372
DOI: 10.1016/j.bbrc.2018.07.121

実験手法

X-RAY DIFFRACTION (3.005 Å)