5ZBK

Crystal structure of type-I LOG from Pseudomonas aeruginosa PAO1 in complex with AMP

5ZBK の概要

• エントリーDOI: 10.2210/pdb5zbk/pdb
• 分子名称: Putative cytokinin riboside 5'-monophosphate phosphoribohydrolase, GLYCEROL, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22352.41

構造登録者

Seo, H.,Kim, K.-J. (登録日: 2018-02-12, 公開日: 2018-06-27, 最終更新日: 2023-11-22)

主引用文献

Seo, H.,Kim, K.J.
Structural insight into molecular mechanism of cytokinin activating protein from Pseudomonas aeruginosa PAO1.
Environ. Microbiol., 20:3214-3223, 2018

PubMed Abstract: Cytokinin (CK)-activating enzyme, called LOG, is a phosphoribohydrolase that hydrolyzes nucleotides into nucleobases and phosphoriboses. This reaction is a fascinating target for regulation of cellular active CK. However, misannotation of LOG as a lysine decarboxylase and the lack of detailed catalytic and substrate-binding mechanisms have prevented studies of LOG at a protein-level. In this study, we determined the crystal structure of PA4923 from Pseudomonas aeruginosa PAO1. The overall structure of PA4923 resembles those of type-I LOGs, and it exhibited phosphoribohydrolase activity against AMP. These observations indicated that PA4923 functions as an LOG. We also determined the PaLOG structure in complex with AMP and elucidated the detailed binding mode of LOG against the AMP substrate. Interestingly, PaLOG undergoes an open/closed conformational change upon binding AMP, during which the Glu74 residue located on the β3-β4 connecting loop flips 180° and moves 13 Å towards the AMP molecule. Structural and amino acid sequence comparisons of LOGs suggest that this conformational change upon substrate binding might be a common phenomenon in LOGs. In addition, based on our structural studies and the reported catalytic mechanism of nucleoside hydrolases, we proposed a catalytic mechanism for LOG in which an oxocarbenium ion-like transition state is formed.

PubMed: 29901273
DOI: 10.1111/1462-2920.14287

実験手法

X-RAY DIFFRACTION (2.3 Å)