5Z8Y

Crystal structure of human LRRTM2 in complex with Neurexin 1beta

Summary for 5Z8Y

• Entry DOI: 10.2210/pdb5z8y/pdb
• Descriptor: Leucine-rich repeat transmembrane neuronal protein 2, Neurexin-1-beta, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: synapse, leucine-rich repeat, synaptic organizer, cell adhesion
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 8
• Total formula weight: 247934.97

Authors

Yamagata, A.,Fukai, S. (deposition date: 2018-02-01, release date: 2018-10-10, Last modification date: 2023-11-22)

Primary citation

Yamagata, A.,Goto-Ito, S.,Sato, Y.,Shiroshima, T.,Maeda, A.,Watanabe, M.,Saitoh, T.,Maenaka, K.,Terada, T.,Yoshida, T.,Uemura, T.,Fukai, S.
Structural insights into modulation and selectivity of transsynaptic neurexin-LRRTM interaction.
Nat Commun, 9:3964-3964, 2018

PubMed Abstract: Leucine-rich repeat transmembrane neuronal proteins (LRRTMs) function as postsynaptic organizers that induce excitatory synapses. Neurexins (Nrxns) and heparan sulfate proteoglycans have been identified as presynaptic ligands for LRRTMs. Specifically, LRRTM1 and LRRTM2 bind to the Nrxn splice variant lacking an insert at the splice site 4 (S4). Here, we report the crystal structure of the Nrxn1β-LRRTM2 complex at 3.4 Å resolution. The Nrxn1β-LRRTM2 interface involves Ca-mediated interactions and overlaps with the Nrxn-neuroligin interface. Together with structure-based mutational analyses at the molecular and cellular levels, the present structural analysis unveils the mechanism of selective binding between Nrxn and LRRTM1/2 and its modulation by the S4 insertion of Nrxn.

PubMed: 30262834
DOI: 10.1038/s41467-018-06333-8

Experimental method

X-RAY DIFFRACTION (3.4 Å)