5Z75

Artificial L-threonine 3-dehydrogenase designed by ancestral sequence reconstruction.

Summary for 5Z75

• Entry DOI: 10.2210/pdb5z75/pdb
• Descriptor: Artificial L-threonine 3-dehydrogenase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, PHOSPHATE ION, ... (5 entities in total)
• Functional Keywords: ancestral sequence reconstruction, l-threonine 3-dehydrogenase, oxidoreductase
• Biological source: synthetic construct
• Total number of polymer chains: 4
• Total formula weight: 151937.91

Authors

Nakano, S.,Motoyama, T.,Miyashita, Y.,Ishizuka, Y.,Matsuo, N.,Tokiwa, H.,Shinoda, S.,Asano, Y.,Ito, S. (deposition date: 2018-01-27, release date: 2018-08-22, Last modification date: 2023-11-22)

• Primary citation: Nakano, S.,Motoyama, T.,Miyashita, Y.,Ishizuka, Y.,Matsuo, N.,Tokiwa, H.,Shinoda, S.,Asano, Y.,Ito, S.; Benchmark Analysis of Native and Artificial NAD+-Dependent Enzymes Generated by a Sequence-Based Design Method with or without Phylogenetic Data.; Biochemistry, 57:3722-3732, 2018; PubMed: 29787243; DOI: 10.1021/acs.biochem.8b00339

Experimental method

X-RAY DIFFRACTION (2.1 Å)