5Z65

Crystal structure of porcine aminopeptidase N ectodomain in functional form

5Z65 の概要

• エントリーDOI: 10.2210/pdb5z65/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900017
• 分子名称: Aminopeptidase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Sus scrofa (Pig)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 108207.08

構造登録者

Sun, Y.G.,Li, R.,Jiang, L.G.,Qiao, S.L.,Zhang, G.P. (登録日: 2018-01-22, 公開日: 2019-01-02, 最終更新日: 2024-11-13)

主引用文献

Sun, Y.G.,Li, R.,Jiang, L.,Qiao, S.,Zhi, Y.,Chen, X.X.,Xie, S.,Wu, J.,Li, X.,Deng, R.,Zhang, G.
Characterization of the interaction between recombinant porcine aminopeptidase N and spike glycoprotein of porcine epidemic diarrhea virus.
Int. J. Biol. Macromol., 117:704-712, 2018

PubMed Abstract: Porcine epidemic diarrhea (PED) has caused huge economic losses to the global pork industry. Infection by its causative agent PED virus (PEDV), an Alpha-coronavirus, was previously proven to be mediated by its spike (S) glycoprotein and a cellular receptor porcine aminopeptidase N (pAPN). Interestingly, some recent studies have indicated that pAPN is not a functional receptor for PEDV. To date, there is a lack of a direct evidence for the interaction between pAPN and PEDV S protein in vitro. Here, we prepared pAPN ectodomain and the truncated variants of PEDV S protein in Drosophila S2 cells. These recombinant proteins were homogeneous after purification by metal-affinity and size-exclusion chromatography. We then assayed the purified target proteins through immunogenicity tests, PEDV binding interference assays, circular dichroism (CD) measurements, pAPN activity assay and structural determination, demonstrating that they were biologically functional. Finally, we characterized their interactions by gel filtration chromatography, native-polyacrylamide gel electrophoresis (PAGE) and surface plasmon resonance (SPR) analyses. The results showed that their affinities were too low to form complexes, which suggest that pAPN may be controversial as the genuine receptor for PEDV. Therefore, further research needs to be carried out to elucidate the interaction between PEDV and its genuine receptor.

PubMed: 29802920
DOI: 10.1016/j.ijbiomac.2018.05.167

実験手法

X-RAY DIFFRACTION (2.65 Å)