5Z1G

Structure of the Brx1 and Ebp2 complex

5Z1G の概要

• エントリーDOI: 10.2210/pdb5z1g/pdb
• 分子名称: rRNA-processing protein EBP2, Ribosome biogenesis protein BRX1, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: brix domain, pre-60s, pre-ribosome, ribosome
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 80800.07

構造登録者

Zheng, S.,Ye, K. (登録日: 2017-12-26, 公開日: 2018-04-11, 最終更新日: 2024-03-27)

主引用文献

Zhou, D.,Zhu, X.,Zheng, S.,Tan, D.,Dong, M.Q.,Ye, K.
Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate
Protein Cell, 10:120-130, 2019

PubMed Abstract: Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 Å resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits.

PubMed: 29557065
DOI: 10.1007/s13238-018-0526-7

実験手法

X-RAY DIFFRACTION (2.294 Å)