5YVR

Crystal Structure of the H277A mutant of ADH/D1, an archaeal halo-thermophilic Red Sea brine pool alcohol dehydrogenase

5YVR の概要

• エントリーDOI: 10.2210/pdb5yvr/pdb
• 関連するPDBエントリー: 5yvm
• 分子名称: alcohol dehydrogenase, MANGANESE (II) ION, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (4 entities in total)
• 機能のキーワード: protein-cofactor complex, dehydrogenase, mutant, halophilic, thermophilic, oxidoreductase
• 由来する生物種: candidate divison MSBL1 archaeon SCGC-AAA259E19
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45102.17

構造登録者

Groetzinger, S.W.,Strillinger, E.,Frank, A.,Eppinger, J.,Groll, M.,Arold, S.T. (登録日: 2017-11-27, 公開日: 2017-12-27, 最終更新日: 2023-11-22)

主引用文献

Groetzinger, S.W.,Karan, R.,Strillinger, E.,Bader, S.,Frank, A.,Al Rowaihi, I.S.,Akal, A.,Wackerow, W.,Archer, J.A.,Rueping, M.,Weuster-Botz, D.,Groll, M.,Eppinger, J.,Arold, S.T.
Identification and Experimental Characterization of an Extremophilic Brine Pool Alcohol Dehydrogenase from Single Amplified Genomes
ACS Chem. Biol., 13:161-170, 2018

PubMed Abstract: Because only 0.01% of prokaryotic genospecies can be cultured and in situ observations are often impracticable, culture-independent methods are required to understand microbial life and harness potential applications of microbes. Here, we report a methodology for the production of proteins with desired functions based on single amplified genomes (SAGs) from unculturable species. We use this method to resurrect an alcohol dehydrogenase (ADH/D1) from an uncharacterized halo-thermophilic archaeon collected from a brine pool at the bottom of the Red Sea. Our crystal structure of 5,6-dihydroxy NADPH-bound ADH/D1 combined with biochemical analyses reveal the molecular features of its halo-thermophily, its unique habitat adaptations, and its possible reaction mechanism for atypical oxygen activation. Our strategy offers a general guide for using SAGs as a source for scientific and industrial investigations of "microbial dark matter."

PubMed: 29188989
DOI: 10.1021/acschembio.7b00792

実験手法

X-RAY DIFFRACTION (1.896 Å)