5YSL

Crystal structure of antibody 1H1 Fab

5YSL の概要

• エントリーDOI: 10.2210/pdb5ysl/pdb
• 分子名称: 1H1 heavy chain, 1H1 light chain (2 entities in total)
• 機能のキーワード: fragment, fab fragment, ficin digestion, intact antibody igg1, immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 189265.34

構造登録者

Hu, X.L.,Yang, F.L. (登録日: 2017-11-14, 公開日: 2017-12-27, 最終更新日: 2024-11-13)

主引用文献

Li, X.,Yang, F.,Hu, X.,Tan, F.,Qi, J.,Peng, R.,Wang, M.,Chai, Y.,Hao, L.,Deng, J.,Bai, C.,Wang, J.,Song, H.,Tan, S.,Lu, G.,Gao, G.F.,Shi, Y.,Tian, K.
Two classes of protective antibodies against Pseudorabies virus variant glycoprotein B: Implications for vaccine design.
PLoS Pathog., 13:e1006777-e1006777, 2017

PubMed Abstract: Pseudorabies virus (PRV) belongs to the Herpesviridae family, and is an important veterinary pathogen. Highly pathogenic PRV variants have caused severe epidemics in China since 2011, causing huge economic losses. To tackle the epidemics, we identified a panel of mouse monoclonal antibodies (mAbs) against PRV glycoprotein B (gB) that effectively block PRV infection. Among these 15 mAbs, fourteen of them block PRV entry in a complement-dependent manner. The remaining one, 1H1 mAb, however can directly neutralize the virus independent of complement and displays broad-spectrum neutralizing activities. We further determined the crystal structure of PRV gB and mapped the epitopes of these antibodies on the structure. Interestingly, all the complement-dependent neutralizing antibodies bind gB at the crown region (domain IV). In contrast, the epitope of 1H1 mAb is located at the bottom of domain I, which includes the fusion loops, indicating 1H1 mAb might neutralize the virus by interfering with the membrane fusion process. Our studies demonstrate that gB contains multiple B-cell epitopes in its crown and base regions and that antibodies targeting different epitopes block virus infection through different mechanisms. These findings would provide important clues for antiviral drug design and vaccine development.

PubMed: 29261802
DOI: 10.1371/journal.ppat.1006777

実験手法

X-RAY DIFFRACTION (2.5 Å)