5YNT

Crystal structure of an aromatic prenyltransferase FAMD1 from Fischerella ambigua UTEX 1903

5YNT の概要

• エントリーDOI: 10.2210/pdb5ynt/pdb
• 関連するPDBエントリー: 5YNU 5YNV 5YNW
• 分子名称: aromatic prenyltransferase, DIMETHYLALLYL DIPHOSPHATE, IMIDAZOLE, ... (5 entities in total)
• 機能のキーワード: prenyltransferase, transferase
• 由来する生物種: Fischerella ambigua UTEX 1903
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74769.27

構造登録者

Wang, J.,Liu, W.D.,Chen, C.C.,Guo, R.T. (登録日: 2017-10-25, 公開日: 2018-08-29, 最終更新日: 2024-03-27)

主引用文献

Wang, J.,Chen, C.C.,Yang, Y.,Liu, W.,Ko, T.P.,Shang, N.,Hu, X.,Xie, Y.,Huang, J.W.,Zhang, Y.,Guo, R.T.
Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis.
Biochem. Biophys. Res. Commun., 495:1782-1788, 2018

PubMed Abstract: FamD1 is a novel CloQ/NphB-family indole prenyltransferase which involves in hapalindole-type alkaloid biosynthesis. Here the native FamD1 structure and three protein-ligand complexes are analyzed to investigate the molecular basis of substrate binding and catalysis. FamD1 adopts a typical ABBA architecture of aromatic prenyltransferase, in which the substrate-binding chamber is found in the central β-barrel. The indole-containing acceptor substrate is bound adjacent to the prenyl donor. Based on the complex structures, a catalytic mechanism of FamD1 is proposed. Functional implications on the sister enzyme FamD2 are also discussed.

PubMed: 29229390
DOI: 10.1016/j.bbrc.2017.12.039

実験手法

X-RAY DIFFRACTION (1.79 Å)