5YN3
Crystal structure of xylose isomerase from Piromyces sp. E2
Summary for 5YN3
| Entry DOI | 10.2210/pdb5yn3/pdb |
| Descriptor | Xylose isomerase, MANGANESE (II) ION, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | xylose isomerase, isomerase |
| Biological source | Piromyces sp. (strain E2) |
| Total number of polymer chains | 4 |
| Total formula weight | 204356.74 |
| Authors | Son, H.-F.,Kim, K.-J. (deposition date: 2017-10-24, release date: 2018-05-09, Last modification date: 2023-11-22) |
| Primary citation | Son, H.,Lee, S.M.,Kim, K.J. Crystal Structure and Biochemical Characterization of Xylose Isomerase fromPiromycessp. E2. J. Microbiol. Biotechnol., 28:571-578, 2018 Cited by PubMed Abstract: Biofuel production using lignocellulosic biomass is gaining attention because it can be substituted for fossil fuels without competing with edible resources. However, because does not have a -xylose metabolic pathway, oxidoreductase or isomerase pathways must be introduced to utilize -xylose from lignocellulosic biomass in . To elucidate the biochemical properties of xylose isomerase (XI) from sp. E2 (XI), we determine its crystal structure in complex with substrate mimic glycerol. An amino acid sequence comparison with other reported XIs and the relative activity measurements using five kinds of divalent metal ions confirmed that XI belongs to class II XI. Moreover kinetic analysis of XI was also performed using Mn²⁺, the preferred divalent metal ion for XI. In addition, the substrate-binding mode of XI could be predicted with the substrate mimic glycerol bound to the active site. These studies may provide structural information to enhance -xylose utilization for biofuel production. PubMed: 29385668DOI: 10.4014/jmb.1711.11026 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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