5YN3

Crystal structure of xylose isomerase from Piromyces sp. E2

5YN3 の概要

• エントリーDOI: 10.2210/pdb5yn3/pdb
• 分子名称: Xylose isomerase, MANGANESE (II) ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: xylose isomerase, isomerase
• 由来する生物種: Piromyces sp. (strain E2)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 204356.74

構造登録者

Son, H.-F.,Kim, K.-J. (登録日: 2017-10-24, 公開日: 2018-05-09, 最終更新日: 2023-11-22)

主引用文献

Son, H.,Lee, S.M.,Kim, K.J.
Crystal Structure and Biochemical Characterization of Xylose Isomerase fromPiromycessp. E2.
J. Microbiol. Biotechnol., 28:571-578, 2018

PubMed Abstract: Biofuel production using lignocellulosic biomass is gaining attention because it can be substituted for fossil fuels without competing with edible resources. However, because does not have a -xylose metabolic pathway, oxidoreductase or isomerase pathways must be introduced to utilize -xylose from lignocellulosic biomass in . To elucidate the biochemical properties of xylose isomerase (XI) from sp. E2 (XI), we determine its crystal structure in complex with substrate mimic glycerol. An amino acid sequence comparison with other reported XIs and the relative activity measurements using five kinds of divalent metal ions confirmed that XI belongs to class II XI. Moreover kinetic analysis of XI was also performed using Mn²⁺, the preferred divalent metal ion for XI. In addition, the substrate-binding mode of XI could be predicted with the substrate mimic glycerol bound to the active site. These studies may provide structural information to enhance -xylose utilization for biofuel production.

PubMed: 29385668
DOI: 10.4014/jmb.1711.11026

実験手法

X-RAY DIFFRACTION (2.7 Å)