5YLZ

Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom

5YLZ の概要

• エントリーDOI: 10.2210/pdb5ylz/pdb
• EMDBエントリー: 6684 6839
• 分子名称: Pre-mRNA-splicing factor 8, Pre-mRNA-splicing factor CEF1, Pre-mRNA-splicing factor SYF2, ... (37 entities in total)
• 機能のキーワード: post-catalytic spliceosome (p complex), rna splicing, exon ligation, spliceosome, splicing
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 43
• 化学式量合計: 2285797.78

構造登録者

Wan, R.,Yan, C.,Bai, R.,Lei, J.,Shi, Y. (登録日: 2017-10-20, 公開日: 2018-07-18, 最終更新日: 2024-03-27)

主引用文献

Bai, R.,Yan, C.,Wan, R.,Lei, J.,Shi, Y.
Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae
Cell, 171:1589-1598.e8, 2017

PubMed Abstract: Removal of an intron from a pre-mRNA by the spliceosome results in the ligation of two exons in the post-catalytic spliceosome (known as the P complex). Here, we present a cryo-EM structure of the P complex from Saccharomyces cerevisiae at an average resolution of 3.6 Å. The ligated exon is held in the active site through RNA-RNA contacts. Three bases at the 3' end of the 5' exon remain anchored to loop I of U5 small nuclear RNA, and the conserved AG nucleotides of the 3'-splice site (3'SS) are specifically recognized by the invariant adenine of the branch point sequence, the guanine base at the 5' end of the 5'SS, and an adenine base of U6 snRNA. The 3'SS is stabilized through an interaction with the 1585-loop of Prp8. The P complex structure provides a view on splice junction formation critical for understanding the complete splicing cycle.

PubMed: 29153833
DOI: 10.1016/j.cell.2017.10.038

実験手法

ELECTRON MICROSCOPY (3.6 Å)