5YLX
Integrated illustration of a valid epitope based on the SLA class I structure and tetramer technique could carry forward the development of molecular vaccine in swine species
Summary for 5YLX
| Entry DOI | 10.2210/pdb5ylx/pdb |
| Descriptor | MHC class I antigen, Beta-2-microglobulin, PRRSV-NSP9-TMP9 peptide, ... (4 entities in total) |
| Functional Keywords | mhc, ctl response, tetramer, immune system |
| Biological source | Sus scrofa (Pig) More |
| Total number of polymer chains | 3 |
| Total formula weight | 43885.98 |
| Authors | |
| Primary citation | Pan, X.,Zhang, N.,Wei, X.,Jiang, Y.,Chen, R.,Li, Q.,Liang, R.,Zhang, L.,Ma, L.,Xia, C. Illumination of PRRSV Cytotoxic T Lymphocyte Epitopes by the Three-Dimensional Structure and Peptidome of Swine Lymphocyte Antigen Class I (SLA-I). Front Immunol, 10:2995-2995, 2019 Cited by PubMed Abstract: To investigate CTL epitope applications in swine, SLA-11502-restricted peptide epitopes matching porcine reproductive and respiratory syndrome virus (PRRSV) strains were explored by crystallography, biochemistry, and the specific pathogen-free (SPF) swine experiments. First, nine predicted PRRSV peptides were tested by assembly of the peptide-SLA-11502 (pSLA-11502) complexes, and the crystal structure of the SLA-11502 complex with one peptide (NSP9-TMP9) was determined. The NSP9-TMP9 peptide conformation presented by pSLA-11502 is different from that of the peptides presented by the known pSLA-10401 and pSLA-3hs0202 complexes. Two consecutive Pro residues make the turn between P3 and P4 of NSP9-TMP9 much sharper. The D pocket of pSLA-11502 is unique and is important for peptide binding. Next, the potential SLA-11502-restricted peptide epitopes matching four typical genetic PRRSV strains were identified based on the peptide-binding motif of SLA-11502 determined by structural analysis and alanine scanning of the NSP9-TMP9 peptide. The tetrameric complex of SLA-11502 and NSP9-TMP9 was constructed and examined. Finally, taking NSP9-TMP9 as an example, the CTL immunogenicity of the identified PRRSV peptide epitope was evaluated. The SPF swine expressing the SLA-11502 alleles were divided into three groups: modified live vaccine (MLV), MLV+NSP9-TMP9, and the blank control group. NSP9-TMP9 was determined as a PRRSV CTL epitope with strong immunogenicity by flow cytometry and IFN-γ expression. Our study developed an integrated approach to identify SLA-I-restricted CTL epitopes from various important viruses and is helpful in designing and applying effective peptide-based vaccines for swine. PubMed: 31969884DOI: 10.3389/fimmu.2019.02995 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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