5YKS

Crystal structure of sucrose nonfermenting-related kinase (SNRK)

Summary for 5YKS

• Entry DOI: 10.2210/pdb5yks/pdb
• Descriptor: SNF-related serine/threonine-protein kinase (1 entity in total)
• Functional Keywords: protein kinase, uba domain, transferase
• Biological source: Homo sapiens (Human)
• Cellular location: Nucleus : Q9NRH2
• Total number of polymer chains: 2
• Total formula weight: 85894.37

Authors

Wang, Y.L.,Wang, J.,Wu, J.W. (deposition date: 2017-10-16, release date: 2017-11-08, Last modification date: 2023-11-22)

Primary citation

Wang, Y.L.,Wang, J.,Chen, X.,Wang, Z.X.,Wu, J.W.
Crystal structure of the kinase and UBA domains of SNRK reveals a distinct UBA binding mode in the AMPK family.
Biochem. Biophys. Res. Commun., 495:1-6, 2018

PubMed Abstract: Sucrose non-fermenting (Snf1)-related kinase (SNRK) is a novel member of the AMP-activated protein kinase (AMPK) family and is involved in many metabolic processes. Here we report the crystal structure of an N-terminal SNRK fragment containing kinase and adjacent ubiquitin-associated (UBA) domains. This structure shows that the UBA domain binds between the N- and C-lobes of the kinase domain. The mode of UBA binding in SNRK largely resembles that in AMPK and brain specific kinase (BRSK), however, unique interactions play vital roles in stabilizing the KD-UBA interface of SNRK. We further propose a potential role of the UBA domain in the regulation of SNRK kinase activity. This study provides new insights into the structural diversities of the AMPK kinase family.

PubMed: 29061304
DOI: 10.1016/j.bbrc.2017.10.105

Experimental method

X-RAY DIFFRACTION (2.9 Å)