5YIV

Caulobacter crescentus GcrA DNA-binding domain(DBD) in complex with methylated dsDNA(crystal form 1)

5YIV の概要

• エントリーDOI: 10.2210/pdb5yiv/pdb
• 分子名称: Cell cycle regulatory protein GcrA, DNA (5'-D(*CP*CP*TP*GP*(6MA)P*TP*TP*CP*G)-3'), DNA (5'-D(*CP*CP*GP*(6MA)P*AP*TP*CP*AP*G)-3'), ... (4 entities in total)
• 機能のキーワード: caulobacter crescentus, gcra, dna-binding domain, transcription factor, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Caulobacter crescentus (strain NA1000 / CB15N); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 43107.40

構造登録者

Wu, X.,Zhang, Y. (登録日: 2017-10-06, 公開日: 2018-03-21, 最終更新日: 2023-11-22)

主引用文献

Wu, X.,Haakonsen, D.L.,Sanderlin, A.G.,Liu, Y.J.,Shen, L.,Zhuang, N.,Laub, M.T.,Zhang, Y.
Structural insights into the unique mechanism of transcription activation by Caulobacter crescentus GcrA.
Nucleic Acids Res., 46:3245-3256, 2018

PubMed Abstract: Canonical bacterial transcription activators bind to non-transcribed promoter elements to increase transcription of their target genes. Here we report crystal structures of binary complexes comprising domains of Caulobacter crescentus GcrA, a noncanonical bacterial transcription factor, that support a novel mechanism for transcription activation through the preferential binding of methylated cis-regulatory elements and the promotion of open complex formation through an interaction with region 2 of the principal σ factor, σ70. We present crystal structures of the C-terminal, σ factor-interacting domain (GcrA-SID) in complex with domain 2 of σ70 (σ702), and the N-terminal, DNA-binding domain (GcrA-DBD) in complex with methylated double-stranded DNA (dsDNA). The structures reveal interactions essential for transcription activation and DNA recognition by GcrA. These structures, along with mutational analyses, support a mechanism of transcription activation in which GcrA associates with RNA polymerase (RNAP) prior to promoter binding through GcrA-SID, arming RNAP with a flexible GcrA-DBD. The RNAP-GcrA complex then binds and activates target promoters harboring a methylated GcrA binding site either upstream or downstream of the transcription start site.

PubMed: 29514271
DOI: 10.1093/nar/gky161

実験手法

X-RAY DIFFRACTION (2.914 Å)