5YHH

Crystal structure of YiiM from Geobacillus stearothermophilus

5YHH の概要

• エントリーDOI: 10.2210/pdb5yhh/pdb
• 分子名称: Uncharacterized conserved protein YiiM (2 entities in total)
• 機能のキーワード: enzyme, mosc, oxidoreductase
• 由来する生物種: Geobacillus stearothermophilus (Bacillus stearothermophilus)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24194.43

構造登録者

Namgung, B.,Kim, J.H.,Song, W.S.,Yoon, S.I. (登録日: 2017-09-28, 公開日: 2018-03-07, 最終更新日: 2023-11-22)

主引用文献

Namgung, B.,Kim, J.H.,Song, W.S.,Yoon, S.I.
Crystal structure of the hydroxylaminopurine resistance protein, YiiM, and its putative molybdenum cofactor-binding catalytic site.
Sci Rep, 8:3304-3304, 2018

PubMed Abstract: The molybdenum cofactor (Moco) is a molybdenum-conjugated prosthetic group that is ubiquitously found in plants, animals, and bacteria. Moco is required for the nitrogen-reducing reaction of the Moco sulfurase C-terminal domain (MOSC) family. Despite the biological significance of MOSC proteins in the conversion of prodrugs and resistance against mutagens, their structural features and Moco-mediated catalysis mechanism have not been described in detail. YiiM is a MOSC protein that is involved in reducing mutagenic 6-N-hydroxylaminopurine to nontoxic adenine in bacteria. Here, we report two crystal structures of YiiM: one from Gram-positive Geobacillus stearothermophilus (gsYiiM) and the other from Gram-negative Escherichia coli (ecYiiM). Although gsYiiM and ecYiiM differ in oligomerization state and protein stability, both consist of three structural modules (a β-barrel and two α-helix bundles) and feature a cavity surrounded by the three modules. The cavity is characterized by positive electrostatic potentials and high sequence conservation. Moreover, the ecYiiM cavity houses a phosphate group, which emulates a part of Moco, and contains a highly reactive invariant cysteine residue. We thus propose that the cavity is the catalytic site where Moco binds and the substrate is reduced. Moreover, our comparative structural analysis highlights the common but distinct structural features of MOSC proteins.

PubMed: 29459651
DOI: 10.1038/s41598-018-21660-y

実験手法

X-RAY DIFFRACTION (2 Å)