5YHF

Crystal structure of SecDF in Super-membrane-facing form

5YHF の概要

• エントリーDOI: 10.2210/pdb5yhf/pdb
• 分子名称: Protein translocase subunit SecDF, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: membrane protein, beta barrel, transport protein, motor protein
• 由来する生物種: Thermus thermophilus
• 細胞内の位置: Cell inner membrane ; Multi- pass membrane protein : Q5SKE6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 82478.58

構造登録者

Tanaka, Y.,Tsukazaki, T.,Yoshikaie, K.,Furukawa, A. (登録日: 2017-09-28, 公開日: 2018-02-14, 最終更新日: 2023-11-22)

主引用文献

Furukawa, A.,Nakayama, S.,Yoshikaie, K.,Tanaka, Y.,Tsukazaki, T.
Remote Coupled Drastic beta-Barrel to beta-Sheet Transition of the Protein Translocation Motor.
Structure, 26:485-489.e2, 2018

PubMed Abstract: The membrane protein SecDF, belonging to the RND superfamily, enhances protein translocation at the extracytoplasmic side using a proton gradient. Here, we report the crystal structure of SecDF in a form we named Super-membrane-facing (Super F) form, demonstrating a β-barrel architecture instead of the previously reported β-sheet structure. Through this structural insight and supporting results of an in vivo crosslinking experiment, we propose a remote coupling model in which a structural change of the transmembrane region drives a functional, extracytoplasmic conformational transition.

PubMed: 29398525
DOI: 10.1016/j.str.2018.01.002

実験手法

X-RAY DIFFRACTION (2.8 Å)