5YFE

Enzymatic and structural characterization of the poly (ethylene terephthalate) hydrolase PETase from I. sakaiensis

5YFE の概要

• エントリーDOI: 10.2210/pdb5yfe/pdb
• 分子名称: Poly(ethylene terephthalate) hydrolase, SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: pet degradation, hydrolase
• 由来する生物種: Ideonella sakaiensis (strain 201-F6)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29069.02

構造登録者

Bao, R.,He, L.H.,Liu, B. (登録日: 2017-09-21, 公開日: 2018-09-26, 最終更新日: 2024-10-16)

主引用文献

Liu, B.,He, L.,Wang, L.,Li, T.,Li, C.,Liu, H.,Luo, Y.,Bao, R.
Protein Crystallography and Site-Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis.
Chembiochem, 2018

PubMed Abstract: Unlike traditional recycling strategies, biodegradation is a sustainable solution for disposing of poly(ethylene terephthalate) (PET) waste. PETase, a newly identified enzyme from Ideonella sakaiensis, has high efficiency and specificity towards PET and is, thus, a prominent candidate for PET degradation. On the basis of biochemical analysis, we propose that a wide substrate-binding pocket is critical for its excellent ability to hydrolyze crystallized PET. Structure-guided site-directed mutagenesis revealed an improvement in PETase catalytic efficiency, providing valuable insight into how the molecular engineering of PETase can optimize its application in biocatalysis.

PubMed: 29603535
DOI: 10.1002/cbic.201800097

実験手法

X-RAY DIFFRACTION (1.39 Å)