5YD8

Crystal structure of human PCNA in complex with APIM of human ZRANB3

5YD8 の概要

• エントリーDOI: 10.2210/pdb5yd8/pdb
• 分子名称: Proliferating cell nuclear antigen, ZRANB3 (3 entities in total)
• 機能のキーワード: complex, dna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 90186.79

構造登録者

Hashimoto, H.,Tagata, R. (登録日: 2017-09-12, 公開日: 2018-04-18, 最終更新日: 2024-10-16)

主引用文献

Hara, K.,Uchida, M.,Tagata, R.,Yokoyama, H.,Ishikawa, Y.,Hishiki, A.,Hashimoto, H.
Structure of proliferating cell nuclear antigen (PCNA) bound to an APIM peptide reveals the universality of PCNA interaction.
Acta Crystallogr.,Sect.F, 74:214-221, 2018

PubMed Abstract: Proliferating cell nuclear antigen (PCNA) provides a molecular platform for numerous protein-protein interactions in DNA metabolism. A large number of proteins associated with PCNA have a well characterized sequence termed the PCNA-interacting protein box motif (PIPM). Another PCNA-interacting sequence termed the AlkB homologue 2 PCNA-interacting motif (APIM), comprising the five consensus residues (K/R)-(F/Y/W)-(L/I/V/A)-(L/I/V/A)-(K/R), has also been identified in various proteins. In contrast to that with PIPM, the PCNA-APIM interaction is less well understood. Here, the crystal structure of PCNA bound to a peptide carrying an APIM consensus sequence, RFLVK, was determined and structure-based interaction analysis was performed. The APIM peptide binds to the PIPM-binding pocket on PCNA in a similar way to PIPM. The phenylalanine and leucine residues within the APIM consensus sequence and a hydrophobic residue that precedes the APIM consensus sequence are crucially involved in interactions with the hydrophobic pocket of PCNA. This interaction is essential for overall binding. These results provide a structural basis for regulation of the PCNA interaction and might aid in the development of specific inhibitors of this interaction.

PubMed: 29633969
DOI: 10.1107/S2053230X18003242

実験手法

X-RAY DIFFRACTION (2.3 Å)