5YCO

Complex structure of PCNA with UHRF2

5YCO の概要

• エントリーDOI: 10.2210/pdb5yco/pdb
• 分子名称: Proliferating cell nuclear antigen, E3 ubiquitin-protein ligase UHRF2, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: complex structure, pcna, uhrf2, dna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus : P12004 Q96PU4
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 124603.02

構造登録者

Wu, M.,Chen, W.,Hang, T.,Wang, C.,Zhang, X.,Zang, J. (登録日: 2017-09-07, 公開日: 2017-11-15, 最終更新日: 2025-09-17)

主引用文献

Chen, W.,Wu, M.,Hang, T.,Wang, C.,Zhang, X.,Zang, J.
Structure insights into the molecular mechanism of the interaction between UHRF2 and PCNA.
Biochem. Biophys. Res. Commun., 494:575-580, 2017

PubMed Abstract: UHRF2 (Ubiquitin-like with PHD and ring finger domains 2) is an E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications and cell cycle regulation by interacting with multiple epigenetic or cell-cycle related proteins. Previous studied have identified PCNA (Proliferating cell nuclear antigen) as an interacting partner of UHRF2 by using the antibody microarray. However, the molecular mechanism and the function of UHRF2-PCNA interaction remains unclear. Here, we report the complex structure of PCNA and the peptide (NEILQTLLDLFFPGYSK) derived from UHRF2 that contains a PIP box. Structural analysis combined with mutagenesis experiments provide the molecular basis for the recognition of UHRF2 by PCNA via PIP-box.

PubMed: 28951215
DOI: 10.1016/j.bbrc.2017.09.102

実験手法

X-RAY DIFFRACTION (2.199 Å)