5Y9S

Crystal structure of VV2_1132, a LysR family transcriptional regulator

5Y9S の概要

• エントリーDOI: 10.2210/pdb5y9s/pdb
• 分子名称: VV2_1132, BROMIDE ION (3 entities in total)
• 機能のキーワード: lysr, transcription
• 由来する生物種: Vibrio vulnificus CMCP6
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 138125.64

構造登録者

Jang, Y.,Hong, S.,Jo, I.,Ahn, J.,Ha, N.C. (登録日: 2017-08-28, 公開日: 2018-03-28, 最終更新日: 2024-03-27)

主引用文献

Jang, Y.,Choi, G.,Hong, S.,Jo, I.,Ahn, J.,Choi, S.H.,Ha, N.C.
A Novel Tetrameric Assembly Configuration in VV2_1132, a LysR-Type Transcriptional Regulator inVibrio vulnificus
Mol. Cells, 41:301-310, 2018

PubMed Abstract: LysR-type transcriptional regulators (LTTRs) contain an N-terminal DNA binding domain (DBD) and a C-terminal regulatory domain (RD). Typically, LTTRs function as homotetramers. VV2_1132 was identified in as an LTTR that is a homologue of HypT (also known as YjiE or QseD) in . In this study, we determined the crystal structure of full-length VV2_1132 at a resolution of 2.2 Å, thereby revealing a novel combination of the domains in the tetrameric assembly. Only one DBD dimer in the tetramer can bind to DNA, because the DNA binding motifs of the other DBD dimer are completely buried in the tetrameric assembly. Structural and functional analyses of VV2_1132 suggest that it might not perform the same role as HypT, indicating that further study is required to elucidate the function of this gene in . The unique structure of VV2_1132 extends our knowledge of LTTR function and mechanisms of action.

PubMed: 29487273
DOI: 10.14348/molcells.2018.2190

実験手法

X-RAY DIFFRACTION (2.199 Å)