5Y6K

Human serum trnasferrin bound to a fluorescent probe

5Y6K の概要

• エントリーDOI: 10.2210/pdb5y6k/pdb
• 分子名称: Serotransferrin, MALONATE ION, FE (III) ION, ... (5 entities in total)
• 機能のキーワード: fluorescent, probe, transferrin, metal transport
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Secreted: P02787
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 76519.16

構造登録者

Jiang, N.,Cheng, T.,Wang, M.,Chan, G.C.F.,Jin, L.,Li, H.,Sun, H. (登録日: 2017-08-12, 公開日: 2018-01-24, 最終更新日: 2024-11-13)

主引用文献

Jiang, N.,Cheng, T.,Wang, M.,Chan, G.C.,Jin, L.,Li, H.,Sun, H.
Tracking iron-associated proteomes in pathogens by a fluorescence approach.
Metallomics, 10:77-82, 2018

PubMed Abstract: Porphyromonas gingivalis is a key oral anaerobic bacterium involved in human periodontitis, which may affect up to 15% adults worldwide. Using the membrane permeable fluorescent probe Fe-TRACER, we identified 17 iron-associated proteins in Porphyromonas gingivalis. We demonstrated the specific binding of the probe towards iron-associated proteins using transferrin as an example and provided an X-ray structure of the fluorescent probe-bound transferrin. Our study provides a basis for the understanding of iron homeostasis in pathogens, and our approach based on the integration of fluorescence imaging with proteomics and bioinformatics can be readily extended to mine other metalloproteomes in microbials.

PubMed: 29323384
DOI: 10.1039/c7mt00275k

実験手法

X-RAY DIFFRACTION (2.86 Å)