5Y1A

HBP35 of Porphyromonas gingivalis

Summary for 5Y1A

• Entry DOI: 10.2210/pdb5y1a/pdb
• Descriptor: 35 kDa hemin binding protein (2 entities in total)
• Functional Keywords: porphyromonas gingivalis, beta-sandwich domain, thioredoxin domain, electron transport
• Biological source: Porphyromonas gingivalis
• Total number of polymer chains: 1
• Total formula weight: 37605.39

Authors

Kakuda, S.,Suzuki, M.,Sato, K. (deposition date: 2017-07-20, release date: 2018-07-25, Last modification date: 2024-03-27)

Primary citation

Sato, K.,Kakuda, S.,Yukitake, H.,Kondo, Y.,Shoji, M.,Takebe, K.,Narita, Y.,Naito, M.,Nakane, D.,Abiko, Y.,Hiratsuka, K.,Suzuki, M.,Nakayama, K.
Immunoglobulin-like domains of the cargo proteins are essential for protein stability during secretion by the type IX secretion system.
Mol. Microbiol., 110:64-81, 2018

PubMed Abstract: The periodontal pathogen Porphyromonas gingivalis secretes many potent virulence factors using the type IX secretion system (T9SS). T9SS cargo proteins that have been structurally determined by X-ray crystallography are composed of a signal peptide, functional domain(s), an immunoglobulin (Ig)-like domain and a C-terminal domain. Role of the Ig-like domains of cargo proteins in the T9SS has not been elucidated. Gingipain proteases, which are cargo proteins of the T9SS, were degraded when their Ig-like domains were lacking or truncated. The degradation was dependent on the activity of a quality control factor, HtrA protease. Another T9SS cargo protein, HBP35, which has a thioredoxin domain as a functional domain, was analyzed by X-ray crystallography, revealing that HBP35 has an Ig-like domain after the thioredoxin domain and that the hydrophobic regions of the thioredoxin domain and the Ig-like domain face each other. HBP35 with substitution of hydrophobic amino acids in the Ig-like domain was degraded depending on HtrA. These results suggest that the Ig-like domain mediates stability of the cargo proteins in the T9SS.

PubMed: 30030863
DOI: 10.1111/mmi.14083

Experimental method

X-RAY DIFFRACTION (1.8 Å)