5Y10

SFTSV Gn head domain

5Y10 の概要

• エントリーDOI: 10.2210/pdb5y10/pdb
• 分子名称: Membrane glycoprotein polyprotein, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• 機能のキーワード: sftsv, gn, phlebovirus, bunyavirus, viral protein
• 由来する生物種: Severe fever with thrombocytopenia virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35953.58

構造登録者

Wu, Y.,Gao, F.,Qi, J.X.,Chai, Y.,Gao, G.F. (登録日: 2017-07-19, 公開日: 2017-09-13, 最終更新日: 2024-11-06)

主引用文献

Wu, Y.,Zhu, Y.H.,Gao, F.,Jiao, Y.J.,Oladejo, B.O.,Chai, Y.,Bi, Y.H.,Lu, S.,Dong, M.Q.,Zhang, C.,Huang, G.M.,Wong, G.,Li, N.,Zhang, Y.F.,Li, Y.,Feng, W.H.,Shi, Y.,Liang, M.F.,Zhang, R.G.,Qi, J.X.,Gao, G.F.
Structures of phlebovirus glycoprotein Gn and identification of a neutralizing antibody epitope
Proc. Natl. Acad. Sci. U.S.A., 114:E7564-E7573, 2017

PubMed Abstract: Severe fever with thrombocytopenia syndrome virus (SFTSV) and Rift Valley fever virus (RVFV) are two arthropod-borne phleboviruses in the family, which cause severe illness in humans and animals. Glycoprotein N (Gn) is one of the envelope proteins on the virus surface and is a major antigenic component. Despite its importance for virus entry and fusion, the molecular features of the phleboviruse Gn were unknown. Here, we present the crystal structures of the Gn head domain from both SFTSV and RVFV, which display a similar compact triangular shape overall, while the three subdomains (domains I, II, and III) making up the Gn head display different arrangements. Ten cysteines in the Gn stem region are conserved among phleboviruses, four of which are responsible for Gn dimerization, as revealed in this study, and they are highly conserved for all members in Therefore, we propose an anchoring mode on the viral surface. The complex structure of the SFTSV Gn head and human neutralizing antibody MAb 4-5 reveals that helices α6 in subdomain III is the key component for neutralization. Importantly, the structure indicates that domain III is an ideal region recognized by specific neutralizing antibodies, while domain II is probably recognized by broadly neutralizing antibodies. Collectively, Gn is a desirable vaccine target, and our data provide a molecular basis for the rational design of vaccines against the diseases caused by phleboviruses and a model for bunyavirus Gn embedding on the viral surface.

PubMed: 28827346
DOI: 10.1073/pnas.1705176114

実験手法

X-RAY DIFFRACTION (2.6 Å)