5Y06

Structural characterization of msmeg_4306 from Mycobacterium smegmatis

5Y06 の概要

• エントリーDOI: 10.2210/pdb5y06/pdb
• 分子名称: msmeg_4306, GLYCEROL, ZINC ION, ... (4 entities in total)
• 機能のキーワード: coiled coil helix, zinc ribbon, hypothetical, zinc-sad, unknown function
• 由来する生物種: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29695.48

構造登録者

Kumar, A.,Karthikeyan, S. (登録日: 2017-07-14, 公開日: 2018-05-02, 最終更新日: 2023-11-22)

主引用文献

Kumar, A.,Karthikeyan, S.
Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis
Acta Crystallogr F Struct Biol Commun, 74:166-173, 2018

PubMed Abstract: The MSMEG_4306 gene from Mycobacterium smegmatis encodes a protein of unknown function with 242 amino-acid residues that contains a conserved zinc-ribbon domain at its C-terminus. Here, the crystal structure of MSMEG_4306 determined by the single-wavelength anomalous dispersion method using just one zinc ion co-purified with the protein is reported. The crystal structure of MSMEG_4306 shows a coiled-coil helix domain in the N-terminal region and a zinc-ribbon domain in the C-terminal region. A structural similarity search against the Protein Data Bank using MSMEG_4306 as a query revealed two similar structures, namely CT398 from Chlamydia trachomatis and HP0958 from Helicobacter pylori, although they share only ∼15% sequence identity with MSMEG_4306. Based on comparative analysis, it is predicted that MSMEG_4306 may be involved in secretion systems, possibly by interacting with multiple proteins or nucleic acids.

PubMed: 29497021
DOI: 10.1107/S2053230X18002236

実験手法

X-RAY DIFFRACTION (2.606 Å)