5Y01

Acid-tolerant monomeric GFP, Gamillus, non-fluorescence (OFF) state

5Y01 の概要

• エントリーDOI: 10.2210/pdb5y01/pdb
• 分子名称: Green fluorescent protein, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: fluorescent protein, beta barrel, gfp-like protein, cis-chromophore
• 由来する生物種: Olindias
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30788.11

構造登録者

Nakashima, R.,Sakurai, K.,Shinoda, H.,Matsuda, T.,Nagai, T. (登録日: 2017-07-14, 公開日: 2018-01-17, 最終更新日: 2026-03-18)

主引用文献

Shinoda, H.,Ma, Y.,Nakashima, R.,Sakurai, K.,Matsuda, T.,Nagai, T.
Acid-Tolerant Monomeric GFP from Olindias formosa.
Cell Chem Biol, 25:330-338.e7, 2018

PubMed Abstract: The fluorescent protein (FP) color palette has greatly contributed to the visualization of molecular and cellular processes. However, most FPs lose fluorescence at a pH lower than their neutral pK (∼6), and this has hampered their application in acidic organelles (pH ∼4.5-6.0). Currently, several cyan- and red-colored acid-tolerant FPs are available; however, there are few reports of acid-tolerant green FPs (GFPs) that are practically applicable to bioimaging. Here, we developed the acid-tolerant monomeric GFP "Gamillus" from the jellyfish Olindias formosa, with excellent brightness, maturation speed, and photostability. Results from X-ray crystallography and point mutagenesis suggest that across a broad pH range the acid tolerance is attributed to stabilization of deprotonation in the chromophore phenyl ring by forming a unique trans configuration. We demonstrate that Gamillus can serve as a molecular tag suitable for imaging in acidic organelles through autophagy-mediated molecular tracking to lysosomes.

PubMed: 29290624
DOI: 10.1016/j.chembiol.2017.12.005

実験手法

X-RAY DIFFRACTION (2.65 Å)