5XYW

Crystal structure of drosophila simulans Rhino chromoshadow domain in complex with N-terminal domain

Summary for 5XYW

• Entry DOI: 10.2210/pdb5xyw/pdb
• Descriptor: Rhino, GD21652 (3 entities in total)
• Functional Keywords: chromoshadow, complex, protein binding
• Biological source: Drosophila simulans (Fruit fly); More
• Total number of polymer chains: 4
• Total formula weight: 29127.23

Authors

Yu, B.W.,Huang, Y. (deposition date: 2017-07-10, release date: 2018-06-20, Last modification date: 2024-03-27)

Primary citation

Yu, B.,Lin, Y.A.,Parhad, S.S.,Jin, Z.,Ma, J.,Theurkauf, W.E.,Zhang, Z.Z.,Huang, Y.
Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification
EMBO Rep., 19:-, 2018

PubMed Abstract: PIWI-interacting RNAs (piRNAs) silence transposons in germ cells to maintain genome stability and animal fertility. Rhino, a rapidly evolving heterochromatin protein 1 (HP1) family protein, binds Deadlock in a species-specific manner and so defines the piRNA-producing loci in the genome. Here, we determine the crystal structures of Rhino-Deadlock complex in and In both species, one Rhino binds the N-terminal helix-hairpin-helix motif of one Deadlock protein through a novel interface formed by the beta-sheet in the Rhino chromoshadow domain. Disrupting the interface leads to infertility and transposon hyperactivation in flies. Our structural and functional experiments indicate that electrostatic repulsion at the interaction interface causes cross-species incompatibility between the sibling species. By determining the molecular architecture of this piRNA-producing machinery, we discover a novel HP1-partner interacting mode that is crucial to piRNA biogenesis and transposon silencing. We thus explain the cross-species incompatibility of two sibling species at the molecular level.

PubMed: 29858487
DOI: 10.15252/embr.201745418

Experimental method

X-RAY DIFFRACTION (2.705 Å)