5XYN

The crystal structure of Csm2-Psy3-Shu1-Shu2 complex from budding yeast

5XYN の概要

• エントリーDOI: 10.2210/pdb5xyn/pdb
• 分子名称: Platinum sensitivity protein 3, Chromosome segregation in meiosis protein 2, Suppressor of HU sensitivity involved in recombination protein 1, ... (5 entities in total)
• 機能のキーワード: comlex, replication, dna binding protein
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• 細胞内の位置: Nucleus : Q12318 P38751 P38957; Cytoplasm : P40465
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 96768.33

構造登録者

Zhang, S.,Zhang, T.,Ding, J. (登録日: 2017-07-09, 公開日: 2017-11-08, 最終更新日: 2024-03-27)

主引用文献

Zhang, S.,Wang, L.,Tao, Y.,Bai, T.,Lu, R.,Zhang, T.,Chen, J.,Ding, J.
Structural basis for the functional role of the Shu complex in homologous recombination.
Nucleic Acids Res., 45:13068-13079, 2017

PubMed Abstract: The Shu complex, a conserved regulator consisting of Csm2, Psy3, Shu1 and Shu2 in budding yeast, plays an important role in the assembly of the Rad51-ssDNA filament in homologous recombination. However, the molecular basis for the assembly of the Shu complex and its functional role in DNA repair is still elusive. Here, we report the crystal structure of the yeast Shu complex, revealing that Csm2, Psy3, Shu1 and Shu2 interact with each other in sequence to form a V-shape overall structure. Shu1 adopts a structure resembling the ATPase core domain of Rad51 and represents a new Rad51 paralog. Shu2 assumes a novel structural fold consisting of a conserved zinc-finger containing SWIM domain and a small insertion domain. The functional roles of the key residues are validated using mutagenesis and in vitro pull-down and in vivo yeast growth studies. Structural analysis together with available biological data identifies two potential DNA-binding sites, one of which might be responsible for binding the ssDNA region of the 3'-overhang DNA and the other for the dsDNA region. Collectively, these findings reveal the molecular basis for the assembly of the Shu complex and shed new insight on its functional role in homologous recombination.

PubMed: 29069504
DOI: 10.1093/nar/gkx992

実験手法

X-RAY DIFFRACTION (3.3 Å)