5XXF

Crystal structure of Poz1, Tpz1 and Rap1

5XXF の概要

• エントリーDOI: 10.2210/pdb5xxf/pdb
• 関連するPDBエントリー: 5XXE
• 分子名称: Protection of telomeres protein poz1, Protection of telomeres protein tpz1, Rap1, ... (5 entities in total)
• 機能のキーワード: telomere, sheterin, hub, dna binding protein
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); 詳細
• 細胞内の位置: Cytoplasm : O13852; Chromosome, telomere : O14246
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 70587.73

構造登録者

Xue, J.,Chen, H.,Wu, J.,Lei, M. (登録日: 2017-07-03, 公開日: 2017-12-20, 最終更新日: 2023-11-22)

主引用文献

Xue, J.,Chen, H.,Wu, J.,Takeuchi, M.,Inoue, H.,Liu, Y.,Sun, H.,Chen, Y.,Kanoh, J.,Lei, M.
Structure of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex.
Cell Res., 27:1503-1520, 2017

PubMed Abstract: Telomeric shelterin complex caps chromosome ends and plays a crucial role in telomere maintenance and protection. In the fission yeast Schizosaccharomyces pombe, shelterin is composed of telomeric single- and double-stranded DNA-binding protein subcomplexes Pot1-Tpz1 and Taz1-Rap1, which are bridged by their interacting protein Poz1. However, the structure of Poz1 and how Poz1 functions as an interaction hub in the shelterin complex remain unclear. Here we report the crystal structure of Poz1 in complex with Poz1-binding motifs of Tpz1 and Rap1. The crystal structure shows that Poz1 employs two different binding surfaces to interact with Tpz1 and Rap1. Unexpectedly, the structure also reveals that Poz1 adopts a dimeric conformation. Mutational analyses suggest that proper interactions between Tpz1, Poz1, and Rap1 in the shelterin core complex are required for telomere length homeostasis and heterochromatin structure maintenance at telomeres. Structural resemblance between Poz1 and the TRFH domains of other shelterin proteins in fission yeast and humans suggests a model for the evolution of shelterin proteins.

PubMed: 29160296
DOI: 10.1038/cr.2017.145

実験手法

X-RAY DIFFRACTION (3.1 Å)