5XU1

Structure of a non-canonical ABC transporter from Streptococcus pneumoniae R6

5XU1 の概要

• エントリーDOI: 10.2210/pdb5xu1/pdb
• 分子名称: ABC transporter ATP-binding protein, ABC transporter permeae, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: abc transporter, transport protein
• 由来する生物種: Streptococcus pneumoniae (strain ATCC BAA-255 / R6); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 144862.65

構造登録者

Yang, H.B.,Jiang, Y.L.,Hou, W.T.,Chen, M.T.,Chen, Y.,Zhou, C.Z. (登録日: 2017-06-22, 公開日: 2018-01-24, 最終更新日: 2023-11-22)

主引用文献

Yang, H.B.,Hou, W.T.,Cheng, M.T.,Jiang, Y.L.,Chen, Y.,Zhou, C.Z.
Structure of a MacAB-like efflux pump from Streptococcus pneumoniae.
Nat Commun, 9:196-196, 2018

PubMed Abstract: The spr0693-spr0694-spr0695 operon of Streptococcus pneumoniae encodes a putative ATP-binding cassette (ABC)-type efflux pump involved in the resistance of antibiotics and antimicrobial peptides. Here we report the crystal structures of Spr0694-0695 at 3.3 Å and Spr0693 at 3.0 Å resolution, revealing a MacAB-like efflux pump. The dimeric Spr0694-0695 adopts a non-canonical fold of ABC transporter, the transmembrane domain of which consists of eight tightly packed transmembrane helices with an insertion of extracellular domain between the first and second helices, whereas Spr0693 forms a nanotube channel docked onto the ABC transporter. Structural analyses combined with ATPase activity and antimicrobial susceptibility assays, enable us to propose a putative substrate-entrance tunnel with a lateral access controlled by a guard helix. Altogether, our findings provide structural insights and putative transport mechanism of a MacAB-like efflux pump in Gram-positive bacteria.

PubMed: 29335499
DOI: 10.1038/s41467-017-02741-4

実験手法

X-RAY DIFFRACTION (3.3 Å)