5XTC

Cryo-EM structure of human respiratory complex I transmembrane arm

5XTC の概要

• エントリーDOI: 10.2210/pdb5xtc/pdb
• EMDBエントリー: 6772
• 分子名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial, ... (33 entities in total)
• 機能のキーワード: homo sapiens, oxidoreductase, respiratory, complex, electron transport
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 29
• 化学式量合計: 561076.05

構造登録者

Gu, J.,Wu, M.,Yang, M. (登録日: 2017-06-19, 公開日: 2017-08-30, 最終更新日: 2025-04-09)

主引用文献

Guo, R.,Zong, S.,Wu, M.,Gu, J.,Yang, M.
Architecture of Human Mitochondrial Respiratory Megacomplex I2III2IV2.
Cell, 170:1247-1257.e12, 2017

PubMed Abstract: The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, we examined the human respiratory chain megacomplex-IIIIIV (MCIIIIIV) with 140 subunits and a subset of associated cofactors using cryo-electron microscopy. The MCIIIIIV forms a circular structure with the dimeric CIII located in the center, where it is surrounded by two copies each of CI and CIV. Two cytochrome c (Cyt.c) molecules are positioned to accept electrons on the surface of the c state CIII dimer. Analyses indicate that CII could insert into the gaps between CI and CIV to form a closed ring, which we termed the electron transport chain supercomplex. The structure not only reveals the precise assignment of individual subunits of human CI and CIII, but also enables future in-depth analysis of the electron transport chain as a whole.

PubMed: 28844695
DOI: 10.1016/j.cell.2017.07.050

実験手法

ELECTRON MICROSCOPY (3.7 Å)