5XRS

Crystal structure of A/Minnesota/11/2010 (H3N2) influenza virus hemagglutinin in complex with LSTc

5XRS の概要

• エントリーDOI: 10.2210/pdb5xrs/pdb
• 関連するPDBエントリー: 5w42 5xrq 5xrt
• 分子名称: Hemagglutinin, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (15 entities in total)
• 機能のキーワード: influenza virus, hemagglutinin, human receptor analog, lstc, viral protein
• 由来する生物種: Influenza A virus (A/swine/Minnesota/A01134337/2010(H3N2)); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 247099.83

構造登録者

Zhang, H.,Wilson, I.A. (登録日: 2017-06-09, 公開日: 2018-07-25, 最終更新日: 2022-10-12)

主引用文献

Bangaru, S.,Zhang, H.,Gilchuk, I.M.,Voss, T.G.,Irving, R.P.,Gilchuk, P.,Matta, P.,Zhu, X.,Lang, S.,Nieusma, T.,Richt, J.A.,Albrecht, R.A.,Vanderven, H.A.,Bombardi, R.,Kent, S.J.,Ward, A.B.,Wilson, I.A.,Crowe, J.E.
A multifunctional human monoclonal neutralizing antibody that targets a unique conserved epitope on influenza HA.
Nat Commun, 9:2669-2669, 2018

PubMed Abstract: The high rate of antigenic drift in seasonal influenza viruses necessitates frequent changes in vaccine composition. Recent seasonal H3 vaccines do not protect against swine-origin H3N2 variant (H3N2v) strains that recently have caused severe human infections. Here, we report a human V1-69 gene-encoded monoclonal antibody (mAb) designated H3v-47 that exhibits potent cross-reactive neutralization activity against human and swine H3N2 viruses that circulated since 1989. The crystal structure and electron microscopy reconstruction of H3v-47 Fab with the H3N2v hemagglutinin (HA) identify a unique epitope spanning the vestigial esterase and receptor-binding subdomains that is distinct from that of any known neutralizing antibody for influenza A H3 viruses. MAb H3v-47 functions largely by blocking viral egress from infected cells. Interestingly, H3v-47 also engages Fcγ receptor and mediates antibody dependent cellular cytotoxicity (ADCC). This newly identified conserved epitope can be used in design of novel immunogens for development of broadly protective H3 vaccines.

PubMed: 29991715
DOI: 10.1038/s41467-018-04704-9

実験手法

X-RAY DIFFRACTION (2.907 Å)