5XNP

Crystal structures of human SALM5 in complex with human PTPdelta

5XNP の概要

• エントリーDOI: 10.2210/pdb5xnp/pdb
• 分子名称: Leucine-rich repeat and fibronectin type-III domain-containing protein 5, Receptor-type tyrosine-protein phosphatase delta, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: regulating some neural developments, membrane protein-hydrolase complex, membrane protein/hydrolase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 148135.55

構造登録者

Liu, H.,Lin, Z.,Xu, F. (登録日: 2017-05-24, 公開日: 2018-01-24, 最終更新日: 2024-10-30)

主引用文献

Lin, Z.,Liu, J.,Ding, H.,Xu, F.,Liu, H.
Structural basis of SALM5-induced PTP delta dimerization for synaptic differentiation
Nat Commun, 9:268-268, 2018

PubMed Abstract: SALM5, a synaptic adhesion molecule implicated in autism, induces presynaptic differentiation through binding to the LAR family receptor protein tyrosine phosphatases (LAR-RPTPs) that have been highlighted as presynaptic hubs for synapse formation. The mechanisms underlying SALM5/LAR-RPTP interaction remain unsolved. Here we report crystal structures of human SALM5 LRR-Ig alone and in complex with human PTPδ Ig1-3 (MeA). Distinct from other LAR-RPTP ligands, SALM5 mainly exists as a dimer with LRR domains from two protomers packed in an antiparallel fashion. In the 2:2 heterotetrameric SALM5/PTPδ complex, a SALM5 dimer bridges two separate PTPδ molecules. Structure-guided mutations and heterologous synapse formation assays demonstrate that dimerization of SALM5 is prerequisite for its functionality in inducing synaptic differentiation. This study presents a structural template for the SALM family and reveals a mechanism for how a synaptic adhesion molecule directly induces cis-dimerization of LAR-RPTPs into higher-order signaling assembly.

PubMed: 29348579
DOI: 10.1038/s41467-017-02414-2

実験手法

X-RAY DIFFRACTION (3.729 Å)