5XK6

Structure of a prenyltransferase soaked with IPP

5XK6 の概要

• エントリーDOI: 10.2210/pdb5xk6/pdb
• 関連するPDBエントリー: 5XK3 5XK7 5XK8 5XK9
• 分子名称: Undecaprenyl diphosphate synthase, MAGNESIUM ION, PYROPHOSPHATE 2-, ... (5 entities in total)
• 機能のキーワード: prenyltransferase, antibiotic biosynthesis, transferase
• 由来する生物種: Streptomyces sp. CNH189
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 108370.09

構造登録者

Ko, T.P.,Guo, R.T.,Liu, W.,Chen, C.C.,Gao, J. (登録日: 2017-05-05, 公開日: 2018-01-17, 最終更新日: 2023-11-22)

主引用文献

Gao, J.,Ko, T.P.,Chen, L.,Malwal, S.R.,Zhang, J.,Hu, X.,Qu, F.,Liu, W.,Huang, J.W.,Cheng, Y.S.,Chen, C.C.,Yang, Y.,Zhang, Y.,Oldfield, E.,Guo, R.T.
"Head-to-Middle" and "Head-to-Tail" cis-Prenyl Transferases: Structure of Isosesquilavandulyl Diphosphate Synthase.
Angew. Chem. Int. Ed. Engl., 57:683-687, 2018

PubMed Abstract: We report the first X-ray crystallographic structure of the "head-to-middle" prenyltransferase, isosesquilavandulyl diphosphate synthase, involved in biosynthesis of the merochlorin class of antibiotics. The protein adopts the ζ or cis-prenyl transferase fold but remarkably, unlike tuberculosinol adenosine synthase and other cis-prenyl transferases (e.g. cis-farnesyl, decaprenyl, undecaprenyl diphosphate synthases), the large, hydrophobic side chain does not occupy a central hydrophobic tunnel. Instead, it occupies a surface pocket oriented at 90° to the hydrophobic tunnel. Product chain-length control is achieved by squeezing out the ligand from the conventional allylic S1 binding site, with proton abstraction being achieved using a diphosphate-Asn-Ser relay. The structures revise and unify our thinking as to the mechanism of action of many other prenyl transferases and may also be of use in engineering new merochlorin-class antibiotics.

PubMed: 29215779
DOI: 10.1002/anie.201710185

実験手法

X-RAY DIFFRACTION (1.58 Å)