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5XC3

Crystal structure of Acanthamoeba polyphaga mimivirus Rab GTPase in complex with GDP

5XC3 の概要
エントリーDOI10.2210/pdb5xc3/pdb
関連するPDBエントリー5XC5
分子名称Probable Rab-related GTPase, GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
機能のキーワードrab gtpase, acanthamoeba polyphaga mimivirus, vesicle trafficking, protein transport
由来する生物種Acanthamoeba polyphaga mimivirus (APMV)
細胞内の位置Host cell membrane ; Lipid- anchor ; Cytoplasmic side : Q5UQ27
タンパク質・核酸の鎖数1
化学式量合計20677.46
構造登録者
Ku, B.,You, J.A.,Kim, S.J. (登録日: 2017-03-22, 公開日: 2017-10-25, 最終更新日: 2023-11-22)
主引用文献Ku, B.,You, J.A.,Oh, K.J.,Yun, H.Y.,Lee, H.S.,Shin, H.C.,Jung, J.,Shin, Y.B.,Kim, S.J.
Crystal structures of two forms of the Acanthamoeba polyphaga mimivirus Rab GTPase
Arch. Virol., 162:3407-3416, 2017
Cited by
PubMed Abstract: Acanthamoeba polyphaga mimivirus (APMV) is a member of the family of giant viruses, harboring a 1,200 kbp genome within its 700 nm-diameter viral particle. The R214 gene of the APMV genome was recently shown to encode a homologue of the Rab GTPases, molecular switch proteins known to play a pivotal role in the regulation of membrane trafficking that were considered to exist only in eukaryotes. Herein, we report the first crystal structures of GDP- and GTP-bound forms of APMV Rab GTPase, both of which were determined at high resolution. An in-depth structural comparison of APMV Rab with each other and with mammalian Rab homologues led to an atomic-level elucidation of the inactive-active conformational change upon GDP/GTP exchange. APMV Rab GTPase exhibited considerable structural similarity to human Rab5, as previously predicted based on its amino acid sequence. However, it also contains unique structural features differentiating it from mammalian homologues, such as the functional substitution of a phenylalanine residue for the stabilization of the nucleotide's guanine base.
PubMed: 28779233
DOI: 10.1007/s00705-017-3510-2
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.497 Å)
構造検証レポート
Validation report summary of 5xc3
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-06に公開中

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