5X50

RNA Polymerase II from Komagataella Pastoris (Type-2 crystal)

5X50 の概要

• エントリーDOI: 10.2210/pdb5x50/pdb
• 関連するPDBエントリー: 5X4Z 5X51
• 分子名称: DNA-directed RNA polymerase subunit, RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III, RNA polymerase II subunit B12.5, ... (13 entities in total)
• 機能のキーワード: transcription, rna polymerase, transferase
• 由来する生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast); 詳細
• 細胞内の位置: Nucleus, nucleolus : F2QPE6
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 511005.65

構造登録者

Ehara, H.,Umehara, T.,Sekine, S.,Yokoyama, S. (登録日: 2017-02-14, 公開日: 2017-05-17, 最終更新日: 2023-11-22)

主引用文献

Ehara, H.,Umehara, T.,Sekine, S.I.,Yokoyama, S.
Crystal structure of RNA polymerase II from Komagataella pastoris
Biochem. Biophys. Res. Commun., 487:230-235, 2017

PubMed Abstract: RNA polymerase II (Pol II) is a 12-subunit protein complex that conducts the transcription of mRNA and some small RNAs. In this work, the crystal structure of Pol II from the methylotropic yeast Komagataella pastoris (Pichia pastoris) was determined. While the structure is highly homologous to that of Pol II from the budding yeast Saccharomyces cerevisiae, the stalk and clamp modules of the K. pastoris Pol II displayed large inward rotations, closing the central cleft to a greater extent than in the known S. cerevisiae Pol II structures. The conformational differences reflect the inherent flexibilities of the stalk and the clamp, as additional low-resolution structures of K. pastoris Pol II in different crystal forms revealed diverse stalk and clamp orientations. Comparisons with other eukaryotic/archaeal RNA polymerase structures in the Protein Data Bank revealed the distributions of the stalk and clamp orientations. The conformational plasticity should be essential for transcriptional functions and binding various regulatory factors.

PubMed: 28412353
DOI: 10.1016/j.bbrc.2017.04.039

実験手法

X-RAY DIFFRACTION (4.293 Å)