5X0M

Structure of a eukaryotic voltage-gated sodium channel at near atomic resolution

5X0M の概要

• エントリーDOI: 10.2210/pdb5x0m/pdb
• EMDBエントリー: 6698
• 分子名称: Sodium channel protein, beta-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: ion channel, membrane protein
• 由来する生物種: Periplaneta americana (American cockroach)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 187778.02

構造登録者

Shen, H.,Zhou, Q.,Pan, X.,Li, Z.,Wu, J.,Yan, N. (登録日: 2017-01-21, 公開日: 2017-03-08, 最終更新日: 2024-10-23)

主引用文献

Shen, H.,Zhou, Q.,Pan, X.,Li, Z.,Wu, J.,Yan, N.
Structure of a eukaryotic voltage-gated sodium channel at near-atomic resolution.
Science, 355:-, 2017

PubMed Abstract: Voltage-gated sodium (Na) channels are responsible for the initiation and propagation of action potentials. They are associated with a variety of channelopathies and are targeted by multiple pharmaceutical drugs and natural toxins. Here, we report the cryogenic electron microscopy structure of a putative Na channel from American cockroach (designated NaPaS) at 3.8 angstrom resolution. The voltage-sensing domains (VSDs) of the four repeats exhibit distinct conformations. The entrance to the asymmetric selectivity filter vestibule is guarded by heavily glycosylated and disulfide bond-stabilized extracellular loops. On the cytoplasmic side, a conserved amino-terminal domain is placed below VSD, and a carboxy-terminal domain binds to the III-IV linker. The structure of NaPaS establishes an important foundation for understanding function and disease mechanism of Na and related voltage-gated calcium channels.

PubMed: 28183995
DOI: 10.1126/science.aal4326

実験手法

ELECTRON MICROSCOPY (3.8 Å)