5WYG

The crystal structure of the apo form of Mtb MazF

5WYG の概要

• エントリーDOI: 10.2210/pdb5wyg/pdb
• 分子名称: Probable endoribonuclease MazF7 (2 entities in total)
• 機能のキーワード: mycobacterium tuberculosis, toxin-antitoxin system, mazf, hydrolase
• 由来する生物種: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29303.69

構造登録者

Xie, W.,Chen, R.,Tu, J. (登録日: 2017-01-13, 公開日: 2017-04-12, 最終更新日: 2023-11-22)

主引用文献

Chen, R.,Tu, J.,Liu, Z.,Meng, F.,Ma, P.,Ding, Z.,Yang, C.,Chen, L.,Deng, X.,Xie, W.
Structure of the MazF-mt9 toxin, a tRNA-specific endonuclease from Mycobacterium tuberculosis
Biochem. Biophys. Res. Commun., 486:804-810, 2017

PubMed Abstract: Tuberculosis (TB) is a severe disease caused by Mycobacterium tuberculosis (M. tb) and the well-characterized M. tb MazE/F proteins play important roles in stress adaptation. Recently, the MazF-mt9 toxin has been found to display endonuclease activities towards tRNAs but the mechanism is unknown. We hereby present the crystal structure of apo-MazF-mt9. The enzyme recognizes tRNA with a central UUU motif within the anticodon loop, but is insensitive to the sequence context outside of the loop. Based on our crystallographic and biochemical studies, we identified key residues for catalysis and proposed the potential tRNA-binding site.

PubMed: 28351618
DOI: 10.1016/j.bbrc.2017.03.132

実験手法

X-RAY DIFFRACTION (2.356 Å)