5WYD

Structural of Pseudomonas aeruginosa DspI

5WYD の概要

• エントリーDOI: 10.2210/pdb5wyd/pdb
• 関連するPDBエントリー: 5WYB 5WYC
• 分子名称: Probable enoyl-CoA hydratase/isomerase, PENTANEDIAL, ISOPROPYL ALCOHOL, ... (6 entities in total)
• 機能のキーワード: crotonase, enoyl-coenzyme a (coa) hydratase/isomeras, diffusible signal factor synthesis, isomerase
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 187054.80

構造登録者

Liu, L.,Peng, C.,Li, T.,Li, C.,He, L.,Song, Y.,Zhu, Y.,Shen, Y.,Bao, R. (登録日: 2017-01-12, 公開日: 2018-01-31, 最終更新日: 2023-11-22)

主引用文献

Liu, L.,Li, T.,Cheng, X.J.,Peng, C.T.,Li, C.C.,He, L.H.,Ju, S.M.,Wang, N.Y.,Ye, T.H.,Lian, M.,Xiao, Q.J.,Song, Y.J.,Zhu, Y.B.,Yu, L.T.,Wang, Z.L.,Bao, R.
Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.
Sci Rep, 8:3928-3928, 2018

PubMed Abstract: DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of cis-2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen Pseudomonas aeruginosa (P. aeruginosa). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of dspI results in a remarkable decreased in the pyoverdine production, flagella-dependent swarming motility, and biofilm dispersion as well as attenuated virulence in P. aeruginosa PA14. This study thus unravels the mechanism of DspI in diffusible signal factor (DSF) CDA biosynthesis, providing vital information for developing inhibitors that interfere with DSF associated pathogenicity in P. aeruginosa.

PubMed: 29500457
DOI: 10.1038/s41598-018-22300-1

実験手法

X-RAY DIFFRACTION (2.101 Å)