5WXG

Structure of TAF PHD finger domain binds to H3(1-15)K4ac

Summary for 5WXG

• Entry DOI: 10.2210/pdb5wxg/pdb
• Descriptor: Transcription initiation factor TFIID subunit 3, Histone H3K4ac, ZINC ION, ... (5 entities in total)
• Functional Keywords: taf3, histone h3, hydrolase
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus : Q5VWG9
• Total number of polymer chains: 2
• Total formula weight: 8263.33

Authors

Zhao, S.,Li, H. (deposition date: 2017-01-07, release date: 2017-08-16, Last modification date: 2024-11-20)

Primary citation

Zhao, S.,Yang, M.,Zhou, W.,Zhang, B.,Cheng, Z.,Huang, J.,Zhang, M.,Wang, Z.,Wang, R.,Chen, Z.,Zhu, J.,Li, H.
Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology
Proc. Natl. Acad. Sci. U.S.A., 114:E7245-E7254, 2017

PubMed Abstract: Chemical modifications on histones and DNA/RNA constitute a fundamental mechanism for epigenetic regulation. These modifications often function as docking marks to recruit or stabilize cognate "reader" proteins. So far, a platform for quantitative and high-throughput profiling of the epigenetic interactome is urgently needed but still lacking. Here, we report a 3D-carbene chip-based surface plasmon resonance imaging (SPRi) technology for this purpose. The 3D-carbene chip is suitable for immobilizing versatile biomolecules (e.g., peptides, antibody, DNA/RNA) and features low nonspecific binding, random yet function-retaining immobilization, and robustness for reuses. We systematically profiled binding kinetics of 1,000 histone "reader-mark" pairs on a single 3D-carbene chip and validated two recognition events by calorimetric and structural studies. Notably, a discovery on H3K4me3 recognition by the DNA mismatch repair protein MSH6 in suggests a mechanism of H3K4me3-mediated DNA damage repair in plant.

PubMed: 28808021
DOI: 10.1073/pnas.1704155114

Experimental method

X-RAY DIFFRACTION (1.703 Å)