5WV7

Crystal structure of d(CCGGGGTACCCCGG)2 at 1.4A resolution

5WV7 の概要

• エントリーDOI: 10.2210/pdb5wv7/pdb
• 分子名称: DNA (5'-D(*CP*CP*GP*GP*GP*GP*TP*AP*CP*CP*CP*CP*GP*G)-3') (2 entities in total)
• 機能のキーワード: a-dna duplex, tetragonal space group, co-crystallization with barium chloride., dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 8565.54

構造登録者

Karthik, S.,Thirugnanasambandam, A.,Mandal, P.K.,Gautham, N. (登録日: 2016-12-22, 公開日: 2017-05-24, 最終更新日: 2023-11-22)

主引用文献

Karthik, S.,Thirugnanasambandam, A.,Mandal, P.K.,Gautham, N.
Crystal structure of d(CCGGGGTACCCCGG)2 at 1.4 angstrom resolution.
Acta Crystallogr F Struct Biol Commun, 73:259-265, 2017

PubMed Abstract: The X-ray crystal structure of the DNA tetradecamer sequence d(CCGGGGTACCCCGG) is reported at 1.4 Å resolution in the tetragonal space group P422. The sequence was designed to fold as a four-way junction. However, it forms an A-type double helix in the presence of barium chloride. The metal ion could not be identified in the electron-density map. The crystallographic asymmetric unit consists of one A-type double helix with 12 base pairs per turn, in contrast to 11 base pairs per turn for canonical A-DNA. A large number of solvent molecules have been identified in both the grooves of the duplex and around the backbone phosphate groups.

PubMed: 28471357
DOI: 10.1107/S2053230X17004770

実験手法

X-RAY DIFFRACTION (1.406 Å)