5WQL

Structure of a PDZ-protease bound to a substrate-binding adaptor

Summary for 5WQL

• Entry DOI: 10.2210/pdb5wql/pdb
• Descriptor: Lipoprotein NlpI, Tail-specific protease, ALA-ALA-ALA-ALA-ALA-ALA, ... (6 entities in total)
• Functional Keywords: prc, nlpi, protein degradation, peptidoglycan, protein binding-signaling protein-hydrolase-peptide complex, protein binding/signaling protein/hydrolase/peptide
• Biological source: Escherichia coli K-12; More
• Cellular location: Cell membrane; Lipid-anchor: P0AFB1; Cell inner membrane; Peripheral membrane protein; Periplasmic side: P23865
• Total number of polymer chains: 8
• Total formula weight: 218800.37

Authors

Su, M.Y.,Chang, C.I. (deposition date: 2016-11-27, release date: 2017-11-22, Last modification date: 2023-11-08)

Primary citation

Su, M.Y.,Som, N.,Wu, C.Y.,Su, S.C.,Kuo, Y.T.,Ke, L.C.,Ho, M.R.,Tzeng, S.R.,Teng, C.H.,Mengin-Lecreulx, D.,Reddy, M.,Chang, C.I.
Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc
Nat Commun, 8:1516-1516, 2017

PubMed Abstract: Peptidoglycan (PG) is a highly cross-linked, protective mesh-like sacculus that surrounds the bacterial cytoplasmic membrane. Expansion of PG is tightly coupled to growth of a bacterial cell and requires hydrolases to cleave the cross-links for insertion of nascent PG material. In Escherichia coli, a proteolytic system comprising the periplasmic PDZ-protease Prc and the lipoprotein adaptor NlpI contributes to PG enlargement by regulating cellular levels of MepS, a cross-link-specific hydrolase. Here, we demonstrate how NlpI binds Prc to facilitate the degradation of its substrate MepS by structural and mutational analyses. An NlpI homodimer binds two molecules of Prc and forms three-sided MepS-docking cradles using its tetratricopeptide repeats. Prc forms a monomeric bowl-shaped structure with a lid-like PDZ domain connected by a substrate-sensing hinge that recognizes the bound C terminus of the substrate. In summary, our study reveals mechanistic details of protein degradation by the PDZ-protease Prc bound to its cognate adaptor protein.

PubMed: 29138488
DOI: 10.1038/s41467-017-01697-9

Experimental method

X-RAY DIFFRACTION (2.3 Å)