5WP9

Structural Basis of Mitochondrial Receptor Binding and Constriction by Dynamin-Related Protein 1

これはPDB形式変換不可エントリーです。

5WP9 の概要

• エントリーDOI: 10.2210/pdb5wp9/pdb
• EMDBエントリー: 8874
• 分子名称: Dynamin-1-like protein, Mitochondrial dynamics protein MID49, PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER, ... (4 entities in total)
• 機能のキーワード: mitochondrial division, dynamin related-protein-1, nucleotide, mid49, protein fibril
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 929988.29

構造登録者

Kalia, R.,Wang, R.Y.R.,Yusuf, A.,Thomas, P.V.,Agard, D.A.,Shaw, J.M.,Frost, A. (登録日: 2017-08-03, 公開日: 2018-06-20, 最終更新日: 2024-03-13)

主引用文献

Kalia, R.,Wang, R.Y.,Yusuf, A.,Thomas, P.V.,Agard, D.A.,Shaw, J.M.,Frost, A.
Structural basis of mitochondrial receptor binding and constriction by DRP1.
Nature, 558:401-405, 2018

PubMed Abstract: Mitochondrial inheritance, genome maintenance and metabolic adaptation depend on organelle fission by dynamin-related protein 1 (DRP1) and its mitochondrial receptors. DRP1 receptors include the paralogues mitochondrial dynamics proteins of 49 and 51 kDa (MID49 and MID51) and mitochondrial fission factor (MFF); however, the mechanisms by which these proteins recruit and regulate DRP1 are unknown. Here we present a cryo-electron microscopy structure of full-length human DRP1 co-assembled with MID49 and an analysis of structure- and disease-based mutations. We report that GTP induces a marked elongation and rotation of the GTPase domain, bundle-signalling element and connecting hinge loops of DRP1. In this conformation, a network of multivalent interactions promotes the polymerization of a linear DRP1 filament with MID49 or MID51. After co-assembly, GTP hydrolysis and exchange lead to MID receptor dissociation, filament shortening and curling of DRP1 oligomers into constricted and closed rings. Together, these views of full-length, receptor- and nucleotide-bound conformations reveal how DRP1 performs mechanical work through nucleotide-driven allostery.

PubMed: 29899447
DOI: 10.1038/s41586-018-0211-2

実験手法

ELECTRON MICROSCOPY (4.22 Å)