5WMB

Structure of the 10S (-)-cis-BP-dG modified Rev1 ternary complex (the BP residue is disordered)

5WMB の概要

• エントリーDOI: 10.2210/pdb5wmb/pdb
• 関連するPDBエントリー: 5WM1 5WM8
• 分子名称: DNA (5'-D(*GP*GP*GP*GP*TP*GP*TP*GP*GP*TP*AP*(DDG))-3'), DNA (5'-D(P*AP*TP*CP*GP*CP*TP*AP*CP*CP*AP*CP*AP*CP*CP*C)-3'), DNA repair protein REV1, ... (9 entities in total)
• 機能のキーワード: benzo[a]pyrene rev1 polymerase carcinogen lesion bypass, dna binding protein, transferase-dna complex, transferase/dna
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 59762.07

構造登録者

Rechkoblit, O.,Kolbanovsky, A.,Landes, H.,Geacintov, N.E.,Aggarwal, A.K. (登録日: 2017-07-28, 公開日: 2017-10-25, 最終更新日: 2023-10-04)

主引用文献

Rechkoblit, O.,Kolbanovskiy, A.,Landes, H.,Geacintov, N.E.,Aggarwal, A.K.
Mechanism of error-free replication across benzo[a]pyrene stereoisomers by Rev1 DNA polymerase.
Nat Commun, 8:965-965, 2017

PubMed Abstract: Benzo[a]pyrene (BP) is a carcinogen in cigarette smoke which, after metabolic activation, can react with the exocyclic N amino group of guanine to generate four stereoisomeric BP-N -dG adducts. Rev1 is unique among translesion synthesis DNA polymerases in employing a protein-template-directed mechanism of DNA synthesis opposite undamaged and damaged guanine. Here we report high-resolution structures of yeast Rev1 with three BP-N -dG adducts, namely the 10S (+)-trans-BP-N -dG, 10R (+)-cis-BP-N -dG, and 10S ( - )-cis-BP-N -dG. Surprisingly, in all three structures, the bulky and hydrophobic BP pyrenyl residue is entirely solvent-exposed in the major groove of the DNA. This is very different from the adduct alignments hitherto observed in free or protein-bound DNA. All complexes are well poised for dCTP insertion. Our structures provide a view of cis-BP-N -dG adducts in a DNA polymerase active site, and offer a basis for understanding error-free replication of the BP-derived stereoisomeric guanine adducts.Benzo[a]pyrene (BP) is a carcinogen in cigarette smoke that upon metabolic activation reacts with guanine. Here, the authors present the structures of the translesion DNA synthesis polymerase Rev1 in complex with three of the four possible stereoisomeric BP-N -dG adducts, which gives insights how Rev1 achieves error-free replication.

PubMed: 29042535
DOI: 10.1038/s41467-017-01013-5

実験手法

X-RAY DIFFRACTION (2.25 Å)