5WLG

Crystal Structure of H-2Db with the GAP501 peptide (SQL)

5WLG の概要

• エントリーDOI: 10.2210/pdb5wlg/pdb
• 分子名称: H-2 class I histocompatibility antigen, D-B alpha chain, Beta-2-microglobulin, GAP50 peptide, ... (8 entities in total)
• 機能のキーワード: h-2db, malaria, gap50, immune response gene, tcr, t cell, vb8.1, immune system
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 185102.43

構造登録者

Gras, S.,Farenc, C.,Josephs, T.,Rossjohn, J. (登録日: 2017-07-26, 公開日: 2017-11-15, 最終更新日: 2024-10-23)

主引用文献

Van Braeckel-Budimir, N.,Gras, S.,Ladell, K.,Josephs, T.M.,Pewe, L.,Urban, S.L.,Miners, K.L.,Farenc, C.,Price, D.A.,Rossjohn, J.,Harty, J.T.
A T Cell Receptor Locus Harbors a Malaria-Specific Immune Response Gene.
Immunity, 47:835-847.e4, 2017

PubMed Abstract: Immune response (Ir) genes, originally proposed by Baruj Benacerraf to explain differential antigen-specific responses in animal models, have become synonymous with the major histocompatibility complex (MHC). We discovered a non-MHC-linked Ir gene in a T cell receptor (TCR) locus that was required for CD8 T cell responses to the Plasmodium berghei GAP50 epitope in mice expressing the MHC class I allele H-2D. GAP50-specific CD8 T cell responses emerged from a very large pool of naive Vβ8.1 precursors, which dictated susceptibility to cerebral malaria and conferred protection against recombinant Listeria monocytogenes infection. Structural analysis of a prototypical Vβ8.1 TCR-H-2D-GAP50 ternary complex revealed that germline-encoded complementarity-determining region 1β residues present exclusively in the Vβ8.1 segment mediated essential interactions with the GAP50 peptide. Collectively, these findings demonstrated that Vβ8.1 functioned as an Ir gene that was indispensable for immune reactivity against the malaria GAP50 epitope.

PubMed: 29150238
DOI: 10.1016/j.immuni.2017.10.013

実験手法

X-RAY DIFFRACTION (2.1 Å)