5WLC

The complete structure of the small subunit processome

これはPDB形式変換不可エントリーです。

5WLC の概要

• エントリーDOI: 10.2210/pdb5wlc/pdb
• EMDBエントリー: 8859
• 分子名称: 5' ETS, rpS9_uS4, rpS16_uS9, ... (67 entities in total)
• 機能のキーワード: ribosome assembly, ribosome
• 由来する生物種: Saccharomyces cerevisiae BY4741 (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 70
• 化学式量合計: 4499175.16

構造登録者

Barandun, J.,Chaker-Margot, M.,Hunziker, M.,Klinge, S. (登録日: 2017-07-26, 公開日: 2017-09-27, 最終更新日: 2024-03-13)

主引用文献

Barandun, J.,Chaker-Margot, M.,Hunziker, M.,Molloy, K.R.,Chait, B.T.,Klinge, S.
The complete structure of the small-subunit processome.
Nat. Struct. Mol. Biol., 24:944-953, 2017

PubMed Abstract: The small-subunit processome represents the earliest stable precursor of the eukaryotic small ribosomal subunit. Here we present the cryo-EM structure of the Saccharomyces cerevisiae small-subunit processome at an overall resolution of 3.8 Å, which provides an essentially complete near-atomic model of this assembly. In this nucleolar superstructure, 51 ribosome-assembly factors and two RNAs encapsulate the 18S rRNA precursor and 15 ribosomal proteins in a state that precedes pre-rRNA cleavage at site A1. Extended flexible proteins are employed to connect distant sites in this particle. Molecular mimicry and steric hindrance, as well as protein- and RNA-mediated RNA remodeling, are used in a concerted fashion to prevent the premature formation of the central pseudoknot and its surrounding elements within the small ribosomal subunit.

PubMed: 28945246
DOI: 10.1038/nsmb.3472

実験手法

ELECTRON MICROSCOPY (3.8 Å)