5WFS

70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C4)

This is a non-PDB format compatible entry.

Summary for 5WFS

• Entry DOI: 10.2210/pdb5wfs/pdb
• Related: 5WF0 5WFK
• EMDB information: 8826 8828 8829
• Descriptor: 23S rRNA, 50S ribosomal protein L13, 50S ribosomal protein L14, ... (64 entities in total)
• Functional Keywords: translation, gtpase, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 59
• Total formula weight: 2297549.01

Authors

Fislage, M.,Frank, J. (deposition date: 2017-07-12, release date: 2018-05-02, Last modification date: 2024-03-13)

Primary citation

Fislage, M.,Zhang, J.,Brown, Z.P.,Mandava, C.S.,Sanyal, S.,Ehrenberg, M.,Frank, J.
Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.
Nucleic Acids Res., 46:5861-5874, 2018

PubMed Abstract: The GTPase EF-Tu in ternary complex with GTP and aminoacyl-tRNA (aa-tRNA) promotes rapid and accurate delivery of cognate aa-tRNAs to the ribosomal A site. Here we used cryo-EM to study the molecular origins of the accuracy of ribosome-aided recognition of a cognate ternary complex and the accuracy-amplifying role of the monitoring bases A1492, A1493 and G530 of the 16S rRNA. We used the GTPase-deficient EF-Tu variant H84A with native GTP, rather than non-cleavable GTP analogues, to trap a near-cognate ternary complex in high-resolution ribosomal complexes of varying codon-recognition accuracy. We found that ribosome complexes trapped by GTPase-deficicent ternary complex due to the presence of EF-TuH84A or non-cleavable GTP analogues have very similar structures. We further discuss speed and accuracy of initial aa-tRNA selection in terms of conformational changes of aa-tRNA and stepwise activation of the monitoring bases at the decoding center of the ribosome.

PubMed: 29733411
DOI: 10.1093/nar/gky346

Experimental method

ELECTRON MICROSCOPY (3 Å)