5WFS の概要
| エントリーDOI | 10.2210/pdb5wfs/pdb |
| 関連するPDBエントリー | 5WF0 5WFK |
| EMDBエントリー | 8826 8828 8829 |
| 分子名称 | 23S rRNA, 50S ribosomal protein L13, 50S ribosomal protein L14, ... (64 entities in total) |
| 機能のキーワード | translation, gtpase, ribosome |
| 由来する生物種 | Escherichia coli 詳細 |
| タンパク質・核酸の鎖数 | 59 |
| 化学式量合計 | 2297549.01 |
| 構造登録者 | |
| 主引用文献 | Fislage, M.,Zhang, J.,Brown, Z.P.,Mandava, C.S.,Sanyal, S.,Ehrenberg, M.,Frank, J. Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A. Nucleic Acids Res., 46:5861-5874, 2018 Cited by PubMed Abstract: The GTPase EF-Tu in ternary complex with GTP and aminoacyl-tRNA (aa-tRNA) promotes rapid and accurate delivery of cognate aa-tRNAs to the ribosomal A site. Here we used cryo-EM to study the molecular origins of the accuracy of ribosome-aided recognition of a cognate ternary complex and the accuracy-amplifying role of the monitoring bases A1492, A1493 and G530 of the 16S rRNA. We used the GTPase-deficient EF-Tu variant H84A with native GTP, rather than non-cleavable GTP analogues, to trap a near-cognate ternary complex in high-resolution ribosomal complexes of varying codon-recognition accuracy. We found that ribosome complexes trapped by GTPase-deficicent ternary complex due to the presence of EF-TuH84A or non-cleavable GTP analogues have very similar structures. We further discuss speed and accuracy of initial aa-tRNA selection in terms of conformational changes of aa-tRNA and stepwise activation of the monitoring bases at the decoding center of the ribosome. PubMed: 29733411DOI: 10.1093/nar/gky346 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3 Å) |
構造検証レポート
検証レポート(詳細版)
をダウンロード
をダウンロード
