5WE2

Structural Basis for Telomere Length Regulation by the Shelterin Bridge

5WE2 の概要

• エントリーDOI: 10.2210/pdb5we2/pdb
• 分子名称: Protection of telomeres protein poz1, Protection of telomeres protein tpz1, DNA-binding protein rap1, ... (5 entities in total)
• 機能のキーワード: telomere, shelterin, cooperativity, gene regulation
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); 詳細
• 細胞内の位置: Cytoplasm : O13852; Chromosome, telomere : O14246; Nucleus : Q96TL7
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 70901.89

構造登録者

Kim, J.-K.,Liu, J.,Hu, X.,Sankaran, B.,Qiao, F. (登録日: 2017-07-06, 公開日: 2017-12-20, 最終更新日: 2024-10-16)

主引用文献

Kim, J.K.,Liu, J.,Hu, X.,Yu, C.,Roskamp, K.,Sankaran, B.,Huang, L.,Komives, E.A.,Qiao, F.
Structural Basis for Shelterin Bridge Assembly.
Mol. Cell, 68:698-714.e5, 2017

PubMed Abstract: Telomere elongation through telomerase enables chromosome survival during cellular proliferation. The conserved multifunctional shelterin complex associates with telomeres to coordinate multiple telomere activities, including telomere elongation by telomerase. Similar to the human shelterin, fission yeast shelterin is composed of telomeric sequence-specific double- and single-stranded DNA-binding proteins, Taz1 and Pot1, respectively, bridged by Rap1, Poz1, and Tpz1. Here, we report the crystal structure of the fission yeast Tpz1-Poz1-Rap1 complex that provides the structural basis for shelterin bridge assembly. Biochemical analyses reveal that shelterin bridge assembly is a hierarchical process in which Tpz1 binding to Poz1 elicits structural changes in Poz1, allosterically promoting Rap1 binding to Poz1. Perturbation of the cooperative Tpz1-Poz1-Rap1 assembly through mutation of the "conformational trigger" in Poz1 leads to unregulated telomere lengthening. Furthermore, we find that the human shelterin counterparts TPP1-TIN2-TRF2 also assemble hierarchically, indicating cooperativity as a conserved driving force for shelterin assembly.

PubMed: 29149597
DOI: 10.1016/j.molcel.2017.10.032

実験手法

X-RAY DIFFRACTION (2.5 Å)