5W5Y
RNA polymerase I Initial Transcribing Complex
Summary for 5W5Y
| Entry DOI | 10.2210/pdb5w5y/pdb |
| EMDB information | 8771 8772 8773 8774 8775 8776 8777 |
| Descriptor | DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, ... (21 entities in total) |
| Functional Keywords | rna polymerase i core factor transcription, transcription |
| Biological source | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) More |
| Total number of polymer chains | 20 |
| Total formula weight | 846241.09 |
| Authors | |
| Primary citation | Han, Y.,Yan, C.,Nguyen, T.H.D.,Jackobel, A.J.,Ivanov, I.,Knutson, B.A.,He, Y. Structural mechanism of ATP-independent transcription initiation by RNA polymerase I. Elife, 6:-, 2017 Cited by PubMed Abstract: Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve a structure of Pol I-CF-DNA to 3.8 Å resolution using single-particle cryo-electron microscopy. The structure reveals a bipartite architecture of Core Factor and its recognition of the promoter from -27 to -16. Core Factor's intrinsic mobility correlates well with different conformational states of the Pol I cleft, in addition to the stabilization of either Rrn7 N-terminal domain near Pol I wall or the tandem winged helix domain of A49 at a partially overlapping location. Comparison of the three states in this study with the Pol II system suggests that a ratchet motion of the Core Factor-DNA sub-complex at upstream facilitates promoter melting in an ATP-independent manner, distinct from a DNA translocase actively threading the downstream DNA in the Pol II PIC. PubMed: 28623663DOI: 10.7554/eLife.27414 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.8 Å) |
Structure validation
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